The testicular biopsy procedures are performed under local anaesthetic. The procedure takes about 15 minutes.
Sperm suitable for ICSI are sometimes not found. It is prudent, therefore, to have some form of sperm as “back-up” in order that the ICSI treatment cycles not have to be abandoned.
The sperm recovered by either of these techniques is only suitable for use with ICSI. Once the ICSI has been completed and sperm remaining, of good quality, may be frozen for use with future ICSI cycles.
The success rate for ICSI is typically 25 – 30 % per treatment cycle. However, the success rate is dependant on many factors, such as the age of the woman.

The
vas deferens (plural: vasa deferentia), also called ductus deferens, (Latin: "carrying-away vessel") is part of the male anatomy of some species, including humans.There are two such ducts; they are muscular tubes (surrounded by smooth muscle) connecting the left and right epididymis to the ejaculatory ducts in order to move sperm. Each tube is about 30 centimeters long (in humans).They are part of the spermatic cords.During ejaculation the smooth muscle in the walls of the ductus deferens or vas deferens contracts reflexively, thus propelling the sperm forward. This is also known as peristalsis. The sperm is transferred from the vas deferens into the urethra, collecting secretions from the male accessory sex glands such as the seminal vesicles, prostate gland and the bulbourethral glands, which form the bulk of semen.The vas deferens is supplied by an accompanying artery (artery of vas deferens). This artery normally arises from the superior vesical artery, itself a branch of the internal iliac artery.